Effectiveness of avocado peel extract (Persea americana Mill) on the growth of Porphyromonas gingivalis bacteria

Efektivitas ekstrak kulit alpukat (Persea americana Mill) terhadap pertumbuhan bakteri Porphyromonas gingivalis

Abstract

Porphyromonas gingivalis is a gram-negative anaerobic bacterium involved in the pathogenesis of periodontal disease that can lead to tooth loss. One way to control P.gingivalis is by using chlorhexidine, but continuous use can eliminate the sensation of saltiness on the tongue. Avocado (Persea americana Mill) is one of the natural ingredients that can be utilised as medicinal and antibacterial plants because it contains several secondary metabolite compounds such as flavonoids, tannins, saponins and al-kaloids. The use of avocado peel as an antibacterial P.gingivalis is not yet known for certain so this research was conducted to determine the antibacterial activity of ethanol extract of avocado peel against P.gingivalis. The extract was obtained by extrac-tion using the maceration method, then tested for antibacterial activity of ethanol extract of avocado fruit peel against P.gingi-valis with concentrations of 20%, 40%, 60%, 80%, 100%, chloramphenicol 30 μg/μL as positive control and dimethyl sulfoxide as negative control. This test was conducted using the disc paper diffusion method. It is concluded that the ethanol extract of avocado fruit peel had antibacterial activity against P.gingivalis. The 20% concentration had an average inhibition diameter of 9.92 mm and 100% concentration inhibition of 16.36 mm.